By Nuno M. Neves, Rui L. Reis
In-depth info on usual biomaterials and their purposes for translational medicine!
- Undiluted services: edited through world-leading specialists with contributions from top-notch overseas scientists, collating adventure and state of the art wisdom on common biomaterials from all around the world
- A must-have at the shelf in each biomaterials lab: graduate and PhD scholars starting their occupation in biomaterials technology and skilled researchers and practitioners alike will flip to this complete reference of their day-by-day work
- Link to scientific perform: chapters on translational study make readers conscious of what should be thought of whilst a biomaterial leaves the lab to be frequently used
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Extra resources for Biomaterials from nature for advanced devices and therapies
The cup-shaped porous skeleton serves as a mechanical support and reinforces the hybrid sponge to keep its shape during cell culture and transplantation. The cup-type hybrid porous scaffolds have another effect that the surrounding outside PLLA-collagen sponge cup can protect against cell leakage from the scaffold during cell seeding. The high cell seeding efficiency helps the scaffolds to hold more cells and decreases cell loss. The high porosity provides more space for cell accommodation and tissue formation.
To control the bulk pore structures of large and thick scaffolds, some preparation methods have been developed [26, 27]. Among these methods, the porogen-leaching method offers many advantages for the easy manipulation and control of pore size and porosity. Although the porogen materials can leave replica pores after leaching, they cannot initiate the formation of surrounding pores. As a result, isolated pores are formed in the scaffold, a situation which is not desirable for tissue engineering scaffolds.
3 Collagen recovery After extraction, collagen will be dissolved in the acidic solution and it is necessary to recover it in a dry final form. At least, two different methods are currently used. 01 M NaOH or Residues (rest of samples) Supernatant (ASC) NaCl to a final conc. 1 Scheme of the collagen extraction process, giving fish skins as example. Concentrations and time of reactions are not fixed, they must be adapted to the process. 1 Precipitation with NaCl, dialysis and freeze-drying This is the most used procedure to precipitate collagen.